{"id":19,"date":"2023-01-03T03:44:00","date_gmt":"2023-01-03T03:44:00","guid":{"rendered":"http:\/\/localhost:8888\/website\/gallery\/"},"modified":"2023-02-02T16:37:52","modified_gmt":"2023-02-02T16:37:52","slug":"gallery","status":"publish","type":"page","link":"https:\/\/samarasinghelab.com\/?page_id=19","title":{"rendered":"Research"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"19\" class=\"elementor elementor-19\">\n\t\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-269106e elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"269106e\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-ac994f4\" data-id=\"ac994f4\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5455278 elementor-widget elementor-widget-spacer\" data-id=\"5455278\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.9.2 - 21-12-2022 *\/\n.elementor-column .elementor-spacer-inner{height:var(--spacer-size)}.e-con{--container-widget-width:100%}.e-con-inner>.elementor-widget-spacer,.e-con>.elementor-widget-spacer{width:var(--container-widget-width,var(--spacer-size));--align-self:var(--container-widget-align-self,initial);--flex-shrink:0}.e-con-inner>.elementor-widget-spacer>.elementor-widget-container,.e-con-inner>.elementor-widget-spacer>.elementor-widget-container>.elementor-spacer,.e-con>.elementor-widget-spacer>.elementor-widget-container,.e-con>.elementor-widget-spacer>.elementor-widget-container>.elementor-spacer{height:100%}.e-con-inner>.elementor-widget-spacer>.elementor-widget-container>.elementor-spacer>.elementor-spacer-inner,.e-con>.elementor-widget-spacer>.elementor-widget-container>.elementor-spacer>.elementor-spacer-inner{height:var(--container-widget-height,var(--spacer-size))}<\/style>\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2bae4d7 elementor-widget elementor-widget-heading\" data-id=\"2bae4d7\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.9.2 - 21-12-2022 *\/\n.elementor-heading-title{padding:0;margin:0;line-height:1}.elementor-widget-heading .elementor-heading-title[class*=elementor-size-]>a{color:inherit;font-size:inherit;line-height:inherit}.elementor-widget-heading .elementor-heading-title.elementor-size-small{font-size:15px}.elementor-widget-heading .elementor-heading-title.elementor-size-medium{font-size:19px}.elementor-widget-heading .elementor-heading-title.elementor-size-large{font-size:29px}.elementor-widget-heading .elementor-heading-title.elementor-size-xl{font-size:39px}.elementor-widget-heading .elementor-heading-title.elementor-size-xxl{font-size:59px}<\/style><h2 class=\"elementor-heading-title elementor-size-medium\">Philosophy:<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-df148f8 elementor-widget elementor-widget-text-editor\" data-id=\"df148f8\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.9.2 - 21-12-2022 *\/\n.elementor-widget-text-editor.elementor-drop-cap-view-stacked .elementor-drop-cap{background-color:#818a91;color:#fff}.elementor-widget-text-editor.elementor-drop-cap-view-framed .elementor-drop-cap{color:#818a91;border:3px solid;background-color:transparent}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap{margin-top:8px}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap-letter{width:1em;height:1em}.elementor-widget-text-editor .elementor-drop-cap{float:left;text-align:center;line-height:1;font-size:50px}.elementor-widget-text-editor .elementor-drop-cap-letter{display:inline-block}<\/style>\t\t\t\t<p><span style=\"font-weight: 400; color: #ffffff;\">People first. This is the guiding philosophy for our laboratory. Our greatest assets are the people in our lab, our collaborators, and the innovative ideas and enthusiasm they bring to our science. And, of course, the ultimate goal of our efforts is to help people; our patients with severe neurological disease and the loved ones caring for them.<\/span><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-1a525c4\" data-id=\"1a525c4\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-81f3b7b\" data-id=\"81f3b7b\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-886b6de elementor-widget elementor-widget-image\" data-id=\"886b6de\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.9.2 - 21-12-2022 *\/\n.elementor-widget-image{text-align:center}.elementor-widget-image a{display:inline-block}.elementor-widget-image a img[src$=\".svg\"]{width:48px}.elementor-widget-image img{vertical-align:middle;display:inline-block}<\/style>\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"800\" height=\"792\" src=\"https:\/\/samarasinghelab.com\/wp-content\/uploads\/2023\/01\/resimg2-1024x1014.jpg\" class=\"attachment-large size-large wp-image-304\" alt=\"\" srcset=\"https:\/\/samarasinghelab.com\/wp-content\/uploads\/2023\/01\/resimg2-1024x1014.jpg 1024w, https:\/\/samarasinghelab.com\/wp-content\/uploads\/2023\/01\/resimg2-300x297.jpg 300w, https:\/\/samarasinghelab.com\/wp-content\/uploads\/2023\/01\/resimg2-150x150.jpg 150w, https:\/\/samarasinghelab.com\/wp-content\/uploads\/2023\/01\/resimg2-768x761.jpg 768w, https:\/\/samarasinghelab.com\/wp-content\/uploads\/2023\/01\/resimg2-1536x1522.jpg 1536w, https:\/\/samarasinghelab.com\/wp-content\/uploads\/2023\/01\/resimg2.jpg 1597w\" sizes=\"auto, (max-width: 800px) 100vw, 800px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-4c5d2c3 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"4c5d2c3\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-2056576\" data-id=\"2056576\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-02bbd7a elementor-aspect-ratio-11 elementor-widget elementor-widget-video\" data-id=\"02bbd7a\" data-element_type=\"widget\" data-settings=\"{&quot;video_type&quot;:&quot;hosted&quot;,&quot;aspect_ratio&quot;:&quot;11&quot;,&quot;controls&quot;:&quot;yes&quot;}\" data-widget_type=\"video.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.9.2 - 21-12-2022 *\/\n.elementor-widget-video .elementor-widget-container{overflow:hidden;transform:translateZ(0)}.elementor-widget-video .elementor-open-inline .elementor-custom-embed-image-overlay{position:absolute;top:0;left:0;width:100%;height:100%;background-size:cover;background-position:50%}.elementor-widget-video .elementor-custom-embed-image-overlay{cursor:pointer;text-align:center}.elementor-widget-video .elementor-custom-embed-image-overlay:hover .elementor-custom-embed-play i{opacity:1}.elementor-widget-video .elementor-custom-embed-image-overlay img{display:block;width:100%}.elementor-widget-video .e-hosted-video .elementor-video{-o-object-fit:cover;object-fit:cover}.e-con-inner>.elementor-widget-video,.e-con>.elementor-widget-video{width:var(--container-widget-width);--flex-grow:var(--container-widget-flex-grow)}<\/style>\t\t<div class=\"e-hosted-video elementor-wrapper elementor-fit-aspect-ratio elementor-open-inline\">\n\t\t\t\t\t<video class=\"elementor-video\" src=\"http:\/\/samarasinghelab.com\/wp-content\/uploads\/2023\/01\/media1.mp4\" controls=\"\" controlsList=\"nodownload\"><\/video>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-5522de8\" data-id=\"5522de8\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-fed7249\" data-id=\"fed7249\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-8a24efd elementor-widget elementor-widget-spacer\" data-id=\"8a24efd\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b4d7614 elementor-widget elementor-widget-heading\" data-id=\"b4d7614\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-medium\">Scientific Mission:<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b5568cb elementor-widget elementor-widget-text-editor\" data-id=\"b5568cb\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p dir=\"ltr\"><span style=\"color: #ffffff;\"><span style=\"font-weight: 400;\">We have developed human stem cell derived brain organoid models that are capable of generating complex physiological activities together with tools to record and quantify these. The core <\/span><i><span style=\"font-weight: 400;\">scientific mission<\/span><\/i><span style=\"font-weight: 400;\"> of this laboratory is to continue to innovate and expand on what we have already developed while leveraging our current methodologies to dive deeper into the mechanisms of normal neural circuit development and how neurological disease perturbs circuit formation and function.<\/span><\/span><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-7809d4f elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"7809d4f\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-948575c\" data-id=\"948575c\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6d543fb elementor-widget elementor-widget-spacer\" data-id=\"6d543fb\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9396564 elementor-widget elementor-widget-heading\" data-id=\"9396564\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-medium\">Overview:<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3ed695d elementor-widget elementor-widget-text-editor\" data-id=\"3ed695d\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p><span style=\"color: #ffffff;\"><span style=\"font-weight: 400;\">In all projects described below we primarily use organoids generated from iPSCs harboring single gene variants associated with epilepsy and autism. More recently, we have also acquired multiple iPSC lines harboring pathogenic mutations in the <\/span><i><span style=\"font-weight: 400;\">microtuble-associated protein tau (MAPT)<\/span><\/i><span style=\"font-weight: 400;\"> gene. Patients with this mutation have an early onset Alzheimer\u2019s-like dementia, and we are actively generating organoids harboring this mutation in our studies of hippocampal circuit formation and function.<\/span><\/span><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-a231d1f\" data-id=\"a231d1f\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-6a81d0e\" data-id=\"6a81d0e\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-9d5c89f elementor-widget elementor-widget-image\" data-id=\"9d5c89f\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"800\" height=\"800\" src=\"https:\/\/samarasinghelab.com\/wp-content\/uploads\/2023\/01\/resimg3-1024x1024.png\" class=\"attachment-large size-large wp-image-305\" alt=\"\" srcset=\"https:\/\/samarasinghelab.com\/wp-content\/uploads\/2023\/01\/resimg3-1024x1024.png 1024w, https:\/\/samarasinghelab.com\/wp-content\/uploads\/2023\/01\/resimg3-300x300.png 300w, https:\/\/samarasinghelab.com\/wp-content\/uploads\/2023\/01\/resimg3-150x150.png 150w, https:\/\/samarasinghelab.com\/wp-content\/uploads\/2023\/01\/resimg3-768x768.png 768w, https:\/\/samarasinghelab.com\/wp-content\/uploads\/2023\/01\/resimg3-1536x1536.png 1536w, https:\/\/samarasinghelab.com\/wp-content\/uploads\/2023\/01\/resimg3.png 1608w\" sizes=\"auto, (max-width: 800px) 100vw, 800px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-7ca7939 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"7ca7939\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-ea70e96\" data-id=\"ea70e96\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6bf2ec5 elementor-widget elementor-widget-heading\" data-id=\"6bf2ec5\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-medium\">Examples:<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b8210e3 elementor-widget elementor-widget-text-editor\" data-id=\"b8210e3\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p><span style=\"font-weight: 400; color: #ffffff;\">The following represent a few (but not all) of the ongoing projects in the lab. We also spend considerable effort within all of our projects in the refinement and development of our organoid generation techniques and analysis approaches.\u00a0<\/span><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-9f5ac94 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"9f5ac94\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-2e1e414\" data-id=\"2e1e414\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-89996a7 elementor-widget elementor-widget-heading\" data-id=\"89996a7\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-medium\">Using brain-region specific organoids to delineate regional divergence in human neural circuit development and function:<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-0090ff9 elementor-widget elementor-widget-text-editor\" data-id=\"0090ff9\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p><span style=\"color: #ffffff;\"><span style=\"font-weight: 400;\">Many neurological disorders <\/span><span style=\"font-weight: 400;\">are associated with impairments in multiple cognitive domains. For example, a patient with a primary diagnosis of autism may also have comorbid epilepsy and intellectual disability. Even in cases where the neurological disorder can be traced to a single gene pathogenic variant it remains unclear if a single upstream pathological driver has shared or unique effects on circuit function in distinct brain regions. This kind of regional divergence may have important implications for both disease pathogenesis and treatment. In recent work, we have focused on the differential impact of single gene variants on the development and function of hippocampal versus forebrain cortical circuits. <\/span><\/span><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-29ed8e0 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"29ed8e0\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-169ea07\" data-id=\"169ea07\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-fd34a17 elementor-widget elementor-widget-image\" data-id=\"fd34a17\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1536\" height=\"503\" src=\"https:\/\/samarasinghelab.com\/wp-content\/uploads\/2023\/01\/researchimg1-1536x503.png\" class=\"attachment-1536x1536 size-1536x1536 wp-image-290\" alt=\"\" srcset=\"https:\/\/samarasinghelab.com\/wp-content\/uploads\/2023\/01\/researchimg1-1536x503.png 1536w, https:\/\/samarasinghelab.com\/wp-content\/uploads\/2023\/01\/researchimg1-300x98.png 300w, https:\/\/samarasinghelab.com\/wp-content\/uploads\/2023\/01\/researchimg1-1024x336.png 1024w, https:\/\/samarasinghelab.com\/wp-content\/uploads\/2023\/01\/researchimg1-768x252.png 768w, https:\/\/samarasinghelab.com\/wp-content\/uploads\/2023\/01\/researchimg1-2048x671.png 2048w\" sizes=\"auto, (max-width: 1536px) 100vw, 1536px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-eeb7cd0 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"eeb7cd0\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-0a34e2e\" data-id=\"0a34e2e\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6031d83 elementor-widget elementor-widget-heading\" data-id=\"6031d83\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-medium\">Oscillatory Failure in Autism:<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a54a3da elementor-widget elementor-widget-text-editor\" data-id=\"a54a3da\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p><span style=\"font-weight: 400; color: #ffffff;\">Brain oscillations are believed to be critical for neural computation and can serve as a biomarker of excitatory- inhibitory (E\/I) balance in neural circuits. Disruptions in oscillatory activity have been observed in patients with autism spectrum disease (ASD). Indeed, oscillatory dysregulation is thought to be a pathophysiological mechanism linking E\/I imbalance with cognitive difficulties in ASD. In particular, a failure to generate gamma oscillations in early life is associated with language, cognitive, and attention deficits characteristic of ASDs. We recently developed a novel brain organoid platform ideal for modeling the effect of E\/I imbalance on downstream cellular plasticity and oscillatory activity. Specifically, we have generated an organoid system where we can consistently generate gamma oscillations. In preliminary studies, we have shown a loss of gamma oscillatory power in organoids generated from patient induced pluripotent stem cells (iPSCs) harboring autism risk genes. In this project we will use a combination of multiphoton calcium indicator imaging, confocal microscopy, and single cell RNA sequencing to identify the cellular etiology of this change.<\/span><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-e946e17\" data-id=\"e946e17\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-cd3464f elementor-widget elementor-widget-image\" data-id=\"cd3464f\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/samarasinghelab.com\/wp-content\/uploads\/elementor\/thumbs\/researchimg2-q19jiu39ql1257yepjkijbne5dthvl2xfpehzr5zos.jpg\" title=\"researchimg2\" alt=\"researchimg2\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-4f28f24 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"4f28f24\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-413e032\" data-id=\"413e032\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-1dbf5fa elementor-widget elementor-widget-heading\" data-id=\"1dbf5fa\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-medium\">An Organoid Model of Hippocampal Circuit Function:<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c1c98de elementor-widget elementor-widget-text-editor\" data-id=\"c1c98de\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p><span style=\"color: #ffffff;\"><span style=\"font-weight: 400;\">We have generated hippocampal organoids that are capable of generating stereotyped neural circuit activities associated with learning and memory. Specifically, we have observed patterns of theta-gamma phase amplitude coupling and a specific type of high frequency oscillation called a sharp wave ripple. In ongoing projects, we are exploring how these patterns are altered by single gene pathogenic variants in <\/span><i><span style=\"font-weight: 400;\">tau<\/span><\/i><span style=\"font-weight: 400;\"> that result in an Alzheimer\u2019s-like dementia.<\/span><\/span><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-d02c308 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"d02c308\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-f8a3e3d\" data-id=\"f8a3e3d\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-20cb22d elementor-widget elementor-widget-image\" data-id=\"20cb22d\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1536\" height=\"422\" src=\"https:\/\/samarasinghelab.com\/wp-content\/uploads\/2023\/01\/researchimg3-1536x422.png\" class=\"attachment-1536x1536 size-1536x1536 wp-image-292\" alt=\"\" srcset=\"https:\/\/samarasinghelab.com\/wp-content\/uploads\/2023\/01\/researchimg3-1536x422.png 1536w, https:\/\/samarasinghelab.com\/wp-content\/uploads\/2023\/01\/researchimg3-300x82.png 300w, https:\/\/samarasinghelab.com\/wp-content\/uploads\/2023\/01\/researchimg3-1024x282.png 1024w, https:\/\/samarasinghelab.com\/wp-content\/uploads\/2023\/01\/researchimg3-768x211.png 768w, https:\/\/samarasinghelab.com\/wp-content\/uploads\/2023\/01\/researchimg3-2048x563.png 2048w\" sizes=\"auto, (max-width: 1536px) 100vw, 1536px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Philosophy: People first. This is the guiding philosophy for our laboratory. Our greatest assets are the people in our lab, our collaborators, and the innovative ideas and enthusiasm they bring to our science. And, of course, the ultimate goal of our efforts is to help people; our patients with severe neurological disease and the loved [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"footnotes":""},"class_list":["post-19","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/samarasinghelab.com\/index.php?rest_route=\/wp\/v2\/pages\/19","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/samarasinghelab.com\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/samarasinghelab.com\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/samarasinghelab.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/samarasinghelab.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=19"}],"version-history":[{"count":55,"href":"https:\/\/samarasinghelab.com\/index.php?rest_route=\/wp\/v2\/pages\/19\/revisions"}],"predecessor-version":[{"id":431,"href":"https:\/\/samarasinghelab.com\/index.php?rest_route=\/wp\/v2\/pages\/19\/revisions\/431"}],"wp:attachment":[{"href":"https:\/\/samarasinghelab.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=19"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}